Determination of nitroimidazole antibiotics based on dispersive solid-phase extraction combined with capillary electrophoresis

Jing Yang; Lianfang Chen; Qingying Wang; Xiaolin Mei; Xiupei Yang; Feng Huo

doi:10.1002/elps.202200114

Determination of nitroimidazole antibiotics based on dispersive solid-phase extraction combined with capillary electrophoresis

Electrophoresis. 2023 Apr;44(7-8):634-645. doi: 10.1002/elps.202200114. Epub 2022 Oct 4.

Authors

Jing Yang¹, Lianfang Chen¹, Qingying Wang¹, Xiaolin Mei¹, Xiupei Yang¹, Feng Huo^{1

2}

Affiliations

¹ College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, P. R. China.
² School of Chemistry and Chemical Engineering, Analytical Testing Center, Institute of Micro & Nano Intelligent Sensing, Neijiang Normal University, Neijiang, P. R. China.

PMID: 36153840
DOI: 10.1002/elps.202200114

Abstract

For a long time, the detection of nitroimidazole antibiotics (NIABs) has been a research focus in environmental analytical chemistry. In this work, a novel technique for the analysis of nitroimidazoles was established based on capillary electrophoresis (CE). UiO-66, synthesized using a solvothermal method, was utilized as an adsorbent in the dispersive solid-phase extraction (DSPE) of five different NIABs. The separation and detection of NIABs in environmental water samples were accomplished using the CE diode array detection method. The optimal extraction conditions were obtained after systematically studying the effects of adsorption time, the amount of extractant, and elution solvent on extraction efficiency. According to the results of the study, the limit of detections of the five NIABs were between 16 and 97 ng/mL, the relative standard deviations were between 0.32% and 0.55%, and the spike recoveries were between 87.43% and 104.8%. This study presents a novel technique for measuring NIABs in complex water samples.

Keywords: capillary electrophoresis; dispersive solid-phase extraction; environmental analysis; nitroimidazole antibiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / analysis
Chromatography, High Pressure Liquid
Electrophoresis, Capillary / methods
Nitroimidazoles* / analysis
Solid Phase Extraction / methods
Water
Water Pollutants, Chemical* / analysis

Substances

Anti-Bacterial Agents
Nitroimidazoles
Water Pollutants, Chemical
Water