Detection of biomarkers for filoviral infection with a silicon photonic resonator platform

Krista Meserve; Abraham J Qavi; M Javad Aman; Hong Vu; Larry Zeitlin; John M Dye; Jeffrey W Froude; Daisy W Leung; Lan Yang; Frederick W Holtsberg; Gaya K Amarasinghe; Ryan C Bailey

doi:10.1016/j.xpro.2022.101719

Detection of biomarkers for filoviral infection with a silicon photonic resonator platform

STAR Protoc. 2022 Dec 16;3(4):101719. doi: 10.1016/j.xpro.2022.101719. Epub 2022 Sep 24.

Authors

Affiliations

¹ Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
² Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63130, USA.
³ Integrated BioTherapeutics, Rockville, MD 20850, USA.
⁴ Mapp Biopharmaceutical, Inc., San Diego, CA 92121, USA.
⁵ United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
⁶ United States Army Nuclear and Countering Weapons of Mass Destruction Agency, Fort Belvoir, VA 22060, USA.
⁷ Department of Medicine, Washington University School of Medicine, St Louis, MO 63130, USA.
⁸ Department of Electrical & Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
⁹ Integrated BioTherapeutics, Rockville, MD 20850, USA. Electronic address: rick@integratedbiotherapeutics.com.
¹⁰ Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63130, USA. Electronic address: gamarasinghe@wustl.edu.
¹¹ Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: ryancb@umich.edu.

Abstract

This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).

Keywords: Antibody; Biotechnology and bioengineering; Chemistry; Health sciences.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Biomarkers
Cytokines
Ebolavirus*
Photons
Silicon*

Substances

Silicon
Cytokines
Biomarkers

Abstract

Publication types

MeSH terms

Substances

Grants and funding