An ultra-sensitive rifampicin electrochemical sensor based on Fe3O4 nanoparticles anchored Multiwalled Carbon nanotube modified glassy carbon electrode

Marzie Alizadeh; Padideh Naderi Asrami; Elif Esra Altuner; Fulya Gulbagca; Rima Nour Elhouda Tiri; Aysenur Aygun; İdris Kaynak; Fatih Sen; Somaye Cheraghi

doi:10.1016/j.chemosphere.2022.136566

An ultra-sensitive rifampicin electrochemical sensor based on Fe₃O₄ nanoparticles anchored Multiwalled Carbon nanotube modified glassy carbon electrode

Chemosphere. 2022 Dec;309(Pt 1):136566. doi: 10.1016/j.chemosphere.2022.136566. Epub 2022 Sep 21.

Authors

Marzie Alizadeh¹, Padideh Naderi Asrami², Elif Esra Altuner³, Fulya Gulbagca³, Rima Nour Elhouda Tiri³, Aysenur Aygun³, İdris Kaynak⁴, Fatih Sen⁵, Somaye Cheraghi⁶

Affiliations

¹ Oral and Dental Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
² Massoud Clinical Laboratory, Tehran, Iran.
³ Sen Research Group, Department of Biochemistry, University of Dumlupinar, 43000, Kutahya, Turkiye.
⁴ Department of Machinery and Metal Technologies, University of Usak, 64000, Usak, Turkiye.
⁵ Sen Research Group, Department of Biochemistry, University of Dumlupinar, 43000, Kutahya, Turkiye. Electronic address: fatihsen1980@gmail.com.
⁶ Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran. Electronic address: somayecheragi64@gmail.com.

PMID: 36152837
DOI: 10.1016/j.chemosphere.2022.136566

Abstract

This study aimed to guide future sensor studies against other pharmaceutical drugs by synthesizing Fe₃O₄NPs@MWCNT metallic nanoparticles (NPs). Side damage caused by excessive accumulation of tuberculosis drugs in the body can cause clots in the organs, and cause serious damage such as heart attack and respiratory failure, and threaten human life. Therefore, the development of sensors sensitive to various antibiotics in this study is important for human health. In this study, the sensitivity of Fe₃O₄ NPs to tuberculosis drug (rifampicin) was evaluated by catalytic reaction using bare/GCE, MWCNT/GCE, and Fe₃O₄NPs@MWCNT/GCE electrodes. First of all, Fe₃O₄ NPs were successfully synthesized for the study and MWCNT/GCE and Fe₃O₄ NPs@MWCNT/GCE electrodes were formed with the modification of the MWCNT support material. It was observed that the Fe₃O₄ NPs@MWCNT/GCE electrode gave the highest signal against the other electrodes. The morphological structure of Fe₃O₄ NPs was determined by various characterization techniques such as Transmission Electron Microscopy (TEM), Fourier Transmission Infrared Spectroscopy (FTIR), ultraviolet-visible (UV-Vis), and X-ray differential (XRD) and the obtained NPs were used for sensor studies, and it was observed that the current intensity increased as the scanning speed of each electrode increased in CV and DPV measurements. The average size of Fe₃O₄ NPs was found to be 7.32 ± 3.2 nm. Anodic current peaks occurred in the linear range of 2-25 μM. According to the results obtained from the measurements, the limit of detection (LOD) value was calculated as 0.64 μM limit of quantification (LOQ) 1.92 μM.

Keywords: Fe(3)O(4) NPs; MWCNT; Rifampicin; Sensor; Tuberculosis drug.

MeSH terms

Anti-Bacterial Agents
Electrochemical Techniques / methods
Electrodes
Humans
Metal Nanoparticles* / chemistry
Nanotubes, Carbon* / chemistry
Pharmaceutical Preparations
Rifampin

Substances

Nanotubes, Carbon
Rifampin
Anti-Bacterial Agents
Pharmaceutical Preparations