Bioanode is a key component of bioelectrochemical systems, but the methods characterizing its resistance distribution are lacked. We propose a novel pulse-opencircuit voltammetry (POV) based on the analytical principle clarified from the electron flow pathways of microbe-electrode interfacial processes (MEIPs). A dual-cathode cell is designed to provide an experimental platform for ensuring precise data acquisition of bioanodes. This POV method enables to measure steady state polarization curves and ohmic potential loss curves by integrating potentiostatic discharge and current interruption techniques. They determines reaction resistance (RB,act) and ohmic resistance (RB,ohm) of biofilm with the assistance of impedance spectroscopy measuring material resistance. The results of various bioanodes demonstrate that RB,act is the principal limiting factor and its value relies on catabolism state. Whilst RB,ohm is relevant to extracellular electron transfer behaviors. They are two useful indicators of the dynamic evaluation of biofilm. We anticipate that this method together with the cell platform is accessible to users and has wide applications in bioanode construction and electroactive bacteria investigation.
Keywords: Bioelectrochemistry; Microbe-electrode interfacial processes; Polarization curve; Pulse-opencircuit voltammetry; Resistance distribution.
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