Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry

Abstract

Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as, for instance, epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.

Keywords: Azadibenzocyclooctyne (ADIBO); Catalyst-free; Click chemistry; Immunoassays; Peptide microarrays; Strain-promoted alkyne-azide cycloaddition (SPAAC); c-Myc peptide.