Staining Starch with Iodine Solution

Shengnan Zhao; Yinhui Ren; Cunxu Wei

doi:10.1007/978-1-0716-2675-7_23

Staining Starch with Iodine Solution

Methods Mol Biol. 2023:2566:281-290. doi: 10.1007/978-1-0716-2675-7_23.

Authors

Shengnan Zhao^{1

2}, Yinhui Ren^{1

2}, Cunxu Wei^{3

4}

Affiliations

¹ Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China.
² Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, China.
³ Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China. cxwei@yzu.edu.cn.
⁴ Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, China. cxwei@yzu.edu.cn.

PMID: 36152260
DOI: 10.1007/978-1-0716-2675-7_23

Abstract

Starch is important material in plant tissues, especially for storage tissues. Starches from different plant resources or tissues vary in morphology, content, and physicochemical properties. Starch and iodine can bind specifically to present the shapes and sizes of starch granules in plant tissues. Here, we describe some methods for staining starch in leaf, pollen grain, and starchy seeds with iodine solution. In addition, the isolated starch can also be stained with iodine solution to exhibit its shape and size.

Keywords: Iodine solution; Leaf; Pollen grain; Seed; Starch.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amylose / analysis
Iodine* / analysis
Plants
Pollen
Seeds / chemistry
Staining and Labeling
Starch* / chemistry

Substances

Starch
Amylose
Iodine