Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction

Mariangela Pucci; Claudio D'Addario

doi:10.1007/978-1-0716-2728-0_29

Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction

Methods Mol Biol. 2023:2576:349-359. doi: 10.1007/978-1-0716-2728-0_29.

Authors

Mariangela Pucci¹, Claudio D'Addario²

Affiliations

¹ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
² Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. cdaddario@unite.it.

PMID: 36152201
DOI: 10.1007/978-1-0716-2728-0_29

Abstract

Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), a major development in PCR technology, is a powerful and sensitive gene analysis technique that has revolutionized the field of gene expression assays. In this chapter, we describe in detail RNA extraction, reverse transcription (RT), and relative quantification of genes forming the endocannabinoid system in different experimental models. In particular, we here provide specific and sensitive assays to be used to assess gene expression of the endocannabinoid system components in mouse, rat, or human samples.

Keywords: Endocannabinoid system genes; Fluorescent signals; RNA extraction; Real-time quantitative polymerase chain reaction; Reverse transcription.

MeSH terms

Animals
Endocannabinoids*
Humans
Mice
RNA / metabolism
Rats
Real-Time Polymerase Chain Reaction / methods
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcription*

Substances

Endocannabinoids
RNA