Radiometric Assay of NAPE-PLD Activity

Filomena Fezza; Nicolina Mastrangelo; Mauro Maccarrone

doi:10.1007/978-1-0716-2728-0_18

Radiometric Assay of NAPE-PLD Activity

Methods Mol Biol. 2023:2576:225-232. doi: 10.1007/978-1-0716-2728-0_18.

Authors

Filomena Fezza¹, Nicolina Mastrangelo², Mauro Maccarrone^{3

4}

Affiliations

¹ Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy. filomena.fezza@uniroma2.it.
² Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy.
³ Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
⁴ European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy.

PMID: 36152190
DOI: 10.1007/978-1-0716-2728-0_18

Abstract

N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).

Keywords: Anandamide; Fatty acid amides; N-Acyl-phosphatidylethanolamines-hydrolyzing phospholipase D; N-Acylphosphatidylethanolamines; N-Palmitoylethanolamine; Radioactive assay; Thin layer chromatography.

MeSH terms

Biological Assay
Chromatography, Thin Layer
Phosphatidylethanolamines* / chemistry
Phospholipase D*

Substances

Phosphatidylethanolamines
Phospholipase D