Assay of GTPγS Binding in Autoradiography

Marina Gabaglio; Pamela Prini; Erica Zamberletti; Tiziana Rubino; Daniela Parolaro

doi:10.1007/978-1-0716-2728-0_14

Assay of GTPγS Binding in Autoradiography

Methods Mol Biol. 2023:2576:181-188. doi: 10.1007/978-1-0716-2728-0_14.

Authors

Marina Gabaglio¹, Pamela Prini¹, Erica Zamberletti¹, Tiziana Rubino², Daniela Parolaro³

Affiliations

¹ Department of Biotechnology and Life Sciences, University of Insubria, Busto Arsizio, (VA), Italy.
² Department of Biotechnology and Life Sciences, University of Insubria, Busto Arsizio, (VA), Italy. tiziana.rubino@uninsubria.it.
³ Fondazione Zardi-Gori, Milano, Italy.

PMID: 36152186
DOI: 10.1007/978-1-0716-2728-0_14

Abstract

Autoradiography of radiolabeled GTPγS ([³⁵S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) ex vivo. Here, we describe the protocol for such a method, suitable for investigating CB₁ receptor functionality in tissue slices from rodent brains.

Keywords: Autoradiography; Binding; Brain section; CB1 receptor; G-proteins; [35S]GTPγS.

MeSH terms

Autoradiography
Brain* / metabolism
Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
Receptors, G-Protein-Coupled* / metabolism
Sulfur Radioisotopes / metabolism

Substances

Receptors, G-Protein-Coupled
Sulfur Radioisotopes
Guanosine 5'-O-(3-Thiotriphosphate)