LC-MS/MS Analysis of AEA and 2-AG

Natalia Battista; Federico Fanti; Manuel Sergi

doi:10.1007/978-1-0716-2728-0_4

LC-MS/MS Analysis of AEA and 2-AG

Methods Mol Biol. 2023:2576:41-47. doi: 10.1007/978-1-0716-2728-0_4.

Authors

Natalia Battista¹, Federico Fanti¹, Manuel Sergi²

Affiliations

¹ Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
² Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy. msergi@unite.it.

PMID: 36152176
DOI: 10.1007/978-1-0716-2728-0_4

Abstract

LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.Anandamide (N-arachidonoylethanolamine, AEA) and 2-Arachidonoylglycerol (2-AG) are the most abundant endocannabinoids (eCBs), which play a major role in a wide variety of physiological and pathological processes. Analysis of those compounds by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine AEA and 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy-to-perform extraction procedures, with reduced solvent consumption.

Keywords: 2-AG; AEA; LC–MS/MS; Plasma; Tissue; μSPE.

MeSH terms

Arachidonic Acids
Chromatography, Liquid / methods
Endocannabinoids*
Polyunsaturated Alkamides
Solvents
Tandem Mass Spectrometry* / methods

Substances

Arachidonic Acids
Endocannabinoids
Polyunsaturated Alkamides
Solvents
anandamide