Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry

Karolina Pietrowska; Diana Anna Dmuchowska; Adam Kretowski; Michal Ciborowski

doi:10.1007/978-1-0716-2699-3_15

Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry

Methods Mol Biol. 2023:2571:149-155. doi: 10.1007/978-1-0716-2699-3_15.

Authors

Karolina Pietrowska¹, Diana Anna Dmuchowska², Adam Kretowski¹, Michal Ciborowski³

Affiliations

¹ Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
² Department of Ophthalmology, Medical University of Bialystok, Bialystok, Poland.
³ Metabolomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland. michal.ciborowski@umb.edu.pl.

PMID: 36152159
DOI: 10.1007/978-1-0716-2699-3_15

Abstract

Aqueous humor (AH) is a transparent fluid that fills the anterior segment of the eye. The composition and level of metabolites in AH are important for understanding its physiology and changes caused by the occurrence of eye disease. A simple method for the preparation and analysis of AH samples was developed using the liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) technique. The analyses were performed using two types of chromatography: reversed-phase liquid chromatography-mass spectrometry (LC-RP-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (LC-HILIC-MS), in the sample prepared by one protocol.

Keywords: Aqueous humor; HILIC analysis; LC–MS; Reversed-phase analysis; Untargeted metabolomics.

MeSH terms

Aqueous Humor* / chemistry
Chromatography, Liquid / methods
Chromatography, Reverse-Phase / methods
Hydrophobic and Hydrophilic Interactions
Mass Spectrometry
Metabolomics* / methods