Physico-chemical parameters of phase separation of the mixture of BSA and triton X 100 in aqueous solutions of monohydroxy compounds

Abstract

Clouding behavior and thermodynamic properties for the TX 100 + BSA mixture were investigated in aqueous and aq. alcoholic media. In an aqueous environment, the values of cloud point (CP), at which a clear solution becomes cloudy, for TX 100 decreases with augmentation of the concentration of BSA. The reverse result was obtained in the aq. alcoholic media. In this study, we have used ethanol (EtOH), 1-propanol (1-PrOH), and 2-butanol (2-BuOH) as alcohols. The changes of CP values in alcoholic media have been obtained in the following order: CP_H2O+EtOH > CP_H2O+2-BuOH > CP_H2O+1-PrOH. The standard free energy (∆G_c^o), standard enthalpy (∆H_c^o), and standard entropy (∆S_c^o) changes of clouding were derived at CP. The ΔG_c⁰ values of TX 100 + BSA decreases in the aqueous and alcoholic media with increasing the concentration of BSA and alcohol. This process becomes endothermic in the aq. alcoholic media. Different thermodynamic properties of transfer and entropy-enthalpy compensation parameters for the phase partitioning of the TX 100 + BSA mixture have been calculated and assessed properly.

Keywords: BSA; Clouding phenomena; Hydrophobic interaction; TX 100; Thermodynamic properties.