Antitumor effect of Escherichia coli-derived outer membrane vesicles on neuroblastoma in vitro and in vivo

Liming Jin; Zhaoxia Zhang; Xiaojun Tan; Zhaoying Wang; Bo Tang; Zhang Wang; Mujie Li; Tao Mi; Lianju Shen; Chunlan Long; Guanghui Wei; Dawei He

doi:10.3724/abbs.2022127

Antitumor effect of Escherichia coli-derived outer membrane vesicles on neuroblastoma in vitro and in vivo

Acta Biochim Biophys Sin (Shanghai). 2022 Sep 25;54(9):1301-1313. doi: 10.3724/abbs.2022127.

Authors

Liming Jin^{1

2

3}, Zhaoxia Zhang^{1

2

3}, Xiaojun Tan^{1

2

3}, Zhaoying Wang^{1

2

3}, Bo Tang^{1

2

3}, Zhang Wang^{1

2

3}, Mujie Li^{1

2

3}, Tao Mi^{1

2

3}, Lianju Shen^{2

3}, Chunlan Long^{2

3}, Guanghui Wei^{1

2

3}, Dawei He^{1

2

3}

Affiliations

¹ Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
² Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing 400014, China.
³ China International Science and Technology Cooperation base of Child development and Critical Disorders; National Clinical Research Center for Child Health and Disorders; Ministry of Education Key Laboratory of Child Development and Disorders; Chongqing Key Laboratory of Pediatrics; Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Abstract

Bacterial outer membrane vesicles (OMVs) are spherical microbubbles that contain biological content and are produced by gram-negative bacteria. The use of OMVs as adjuvants for cancer immunotherapy or as drug carriers for targeted therapies has attracted the interest of many scholars. However, it is unclear whether OMVs can exert direct antitumor effects and whether OMVs can inhibit pediatric tumors. Here, we explore the potential of Escherichia coli-derived OMVs to directly suppress neuroblastoma. Our results demonstrate the antitumor effects of OMVs in vitro and in vivo, and no serious adverse reactions were observed. OMV uptake into the cytoplasm and nucleus directly decreases cell stemness, DNA damage, apoptosis and cell cycle arrest, which may be the mechanisms by which OMVs suppress tumors. Our results demonstrate the potential of bacterial OMVs to be used as antitumor adjuvant therapies, increasing the number of candidates for the development of cancer therapies in the future. More relevant studies are urgently needed to demonstrate the efficacy and safety of OMVs.

Keywords: P53; apoptosis; neuroblastoma; outer membrane vesicles.

MeSH terms

Apoptosis
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / metabolism
Child
Escherichia coli* / metabolism
Humans
Neuroblastoma* / drug therapy

Substances

Bacterial Outer Membrane Proteins

Grants and funding

This work was supported by the grant from the Special Project of Technology Innovation and Application Development of Chongqing (No. cstc2019jscx-tjsbX0003).