Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii

Noel-David Nogbou; Dimpho Raesibe Mabela; Buang Matseke; Ntwanano Sipho Mapfumari; Mmammosheledi Elsie Mothibe; Lawrence Chikwelu Obi; Andrew Munyalo Musyoki

doi:10.3390/plants11182374

Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii

Plants (Basel). 2022 Sep 12;11(18):2374. doi: 10.3390/plants11182374.

Authors

Noel-David Nogbou¹, Dimpho Raesibe Mabela², Buang Matseke², Ntwanano Sipho Mapfumari², Mmammosheledi Elsie Mothibe³, Lawrence Chikwelu Obi⁴, Andrew Munyalo Musyoki¹

Affiliations

¹ Department of Microbiological Pathology, School of Medicine, Sefako Makgatho Health Sciences University, P.O. Box 211, Pretoria 0208, South Africa.
² Department of Pharmaceutical Sciences, School of Pharmacy, Sefako Makgatho Health Sciences University, P.O. Box 218, Pretoria 0208, South Africa.
³ Department of Pharmacology, School of Pharmacy, Rhodes University, P.O. Box 94, Grahamstown 6139, South Africa.
⁴ Faculty of Science and Technology, Sefako Makgatho Health Sciences University, P.O Box 138, Pretoria 0204, South Africa.

Abstract

Carbapenemase-producing Acinetobacter baumannii (A. baumannii) is resistant to most of the available antibiotics and poses serious therapeutic challenges. The study investigated Monsonia angustifolia (M. angustifolia) and Momordica balsamina Linn (M. balsamina Linn) extracts for antibacterial activity against a clinical isolate of carbapenemase-producing A. baumannii using the Kirby Bauer disc diffusion and TLC coupled with bioautography. MIC determination experiments were conducted on a molecularly characterized A. baumannii isolate identified using VITEK2. Positive PCR detection of bla_OXA-51 and bla_OXA-23 confirmed isolate identity and the presence of a carbapenemase-encoding gene. Antibacterial activity was observed with the methanolic extract of M. balsamina Linn with a MIC of 0.5 mg/mL. Compounds with Rf values of 0.05; 0.17; 0.39 obtained from M. angustifolia hexane extract; compounds with Rf values of 0.58; 0.78; 0.36; 0.48; 0.5; 0.56; 0.67; 0.9 obtained from M. angustifolia dichloromethane extract; compounds with Rf values of 0.11; 0.56; 0.24; 0.37 obtained from M. angustifolia acetone extract and compounds with Rf values of 0.11; 0.27 obtained from M. angustifolia methanol extract demonstrated a level of antibacterial activity. M. angustifolia and M. balsamina Linn plant extracts have a clinically significant antibacterial activity against a carbapenemase-producing A. baumannii strain.

Keywords: M. angustifolia; M. balsamina Linn; antibacterial activity; carbapenemase-producing A. baumannii; plant extract.

Grants and funding

This research received no external funding.