Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from Euphausia superba

Int J Mol Sci. 2022 Sep 9;23(18):10478. doi: 10.3390/ijms231810478.

Abstract

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a highly conserved enzyme that is involved in glycolysis and gluconeogenesis. In this study, we cloned the fructose-1,6-bisphosphate aldolase gene from Euphausia superba (EsFBA). The full-length cDNA sequence of EsFBA is 1098 bp long and encodes a 365-amino-acid protein. The fructose-1,6-bisphosphate aldolase gene was expressed in Escherichia coli (E. coli). A highly purified protein was obtained using HisTrap HP affinity chromatography and size-exclusion chromatography. The predicted three-dimensional structure of EsFBA showed a 65.66% homology with human aldolase, whereas it had the highest homology (84.38%) with the FBA of Penaeus vannamei. Recombinant EsFBA had the highest activity at 45 °C and pH 7.0 in phosphate buffer. By examining the activity of metal ions and EDTA, we found that the effect of metal ions and EDTA on EsFBA's enzyme activity was not significant, while the presence of borohydride severely reduced the enzymatic activity; thus, EsFBA was confirmed to be a class I aldolase. Furthermore, targeted mutations at positions 34, 147, 188, and 230 confirmed that they are key amino acid residues for EsFBA.

Keywords: Euphausia superba; fructose-1,6-bisphosphate aldolase; heterologous expression; molecular simulation; point mutation.

MeSH terms

  • Aldehyde-Lyases / genetics
  • Amino Acids / metabolism
  • Animals
  • Borohydrides / metabolism
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Edetic Acid / metabolism
  • Escherichia coli / metabolism
  • Euphausiacea*
  • Fructose / metabolism
  • Fructose-Bisphosphate Aldolase* / genetics
  • Fructose-Bisphosphate Aldolase* / metabolism
  • Humans
  • Kinetics
  • Phosphates / metabolism

Substances

  • Amino Acids
  • Borohydrides
  • DNA, Complementary
  • Phosphates
  • Fructose
  • Edetic Acid
  • Aldehyde-Lyases
  • Fructose-Bisphosphate Aldolase

Grants and funding

This research was funded by the Central Public-interest Scientific Institution Basal Research Fund, CAFS(NO.2020TD67); Open Fund Project of Key Lab of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, PRC(2022OPF01).