(1) Background: Ultraviolet-visible (UV-Vis) spectroscopy is a common analytical tool to detect chromophore in compounds by monitoring absorbance spectral wavelengths. Further, it could provide spectral information about complex conjugated systems in mixtures aided by chemometric tools to visualize large UV-Vis datasets as typical in food samples. This review provides novel insight on UV-Vis applications in the last 20 years, as an advanced analytical tool in the quality control of food and dietary supplements, as well as several other applications, including chemotaxonomy, authentication, fingerprinting, and stability studies. (2) Conclusions: A critical assessment of the value of UV application and its novel trends in the quality control (QC) of nutraceuticals reveals the advantages and limitations, focusing on areas where future advancements are in need. Although simple, UV and its novel trends present potential analytical tools with an acceptable error for QC applications from a non-targeted perspective compared to other expensive spectral tools.
Keywords: UV spectroscopy; chemometrics; food analysis; nutraceuticals; quality control.