Mechanism of SCD Participation in Lipid Droplet-Mediated Steroidogenesis in Goose Granulosa Cells

Genes (Basel). 2022 Aug 24;13(9):1516. doi: 10.3390/genes13091516.

Abstract

Stearoyl-CoA desaturase (SCD) is a key enzyme catalyzing the rate-limiting step in monounsaturated fatty acids (MUFAs) production. There may be a mechanism by which SCD is involved in lipid metabolism, which is assumed to be essential for goose follicular development. For this reason, a cellular model of SCD function in goose granulosa cells (GCs) via SCD overexpression and knockdown was used to determine the role of SCD in GC proliferation using flow cytometry. We found that SCD overexpression induced and SCD knockdown inhibited GCs proliferation. Furthermore, ELISA analysis showed that SCD overexpression increased the total cholesterol (TC), progesterone, and estrogen levels in GCs, while SCD knockdown decreased TC, progesterone, and estrogen levels (p < 0.05). Combining these results with those of related multi-omics reports, we proposed a mechanism of SCD regulating the key lipids and differentially expressed gene (DEGs) in glycerophospholipid and glycerolipid metabolism, which participate in steroidogenesis mediated by the lipid droplet deposition in goose GCs. These results add further insights into understanding the lipid metabolism mechanism of goose GCs.

Keywords: SCD; goose granulosa cells; lipid droplets; steroidogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cholesterol / metabolism
  • Estrogens / metabolism
  • Fatty Acids, Monounsaturated
  • Female
  • Geese* / metabolism
  • Glycerophospholipids / metabolism
  • Granulosa Cells / metabolism
  • Lipid Droplets / metabolism
  • Progesterone / metabolism
  • Stearoyl-CoA Desaturase* / genetics
  • Stearoyl-CoA Desaturase* / metabolism

Substances

  • Estrogens
  • Fatty Acids, Monounsaturated
  • Glycerophospholipids
  • Progesterone
  • Cholesterol
  • Stearoyl-CoA Desaturase

Grants and funding

This study is supported by International Cooperation Project (No. 2022YFH0035) funded by Science and Technology Department of Sichuan Province, the Open Fund of Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, the National Natural Science Foundation of China (31972567 and 31802064), the Key Technology Support Program of Sichuan Province (2021YFYZ0014) and the China Agricultural Research System of MOF and MARA (CARS-42-4).