Copper is an important trace element that plays a crucial role in various physiological and biochemical processes in the body. The level of copper content is significantly related to many diseases, so it is very important to establish effective and sensitive methods for copper detection in vitro and vivo. Copper-selective probes have attracted considerable interest in environmental testing and life-process research, but fewer investigations have focused on the luminescence mechanism and bioimaging for Cu2+ detection. In the current study, a novel fluorescein-based A5 fluorescence probe is synthesized and characterized, and the bioimaging performance of the probe is also tested. We observed that the A5 displayed extraordinary selectivity and sensitivity properties to Cu2+ in contrast to other cations in solution. The reaction between A5 and Cu2+ could accelerate the ring-opening process, resulting in a new band at 525 nm during a larger pH range. A good linearity between the fluorescence intensity and concentrations of Cu2+, ranging from 0.1 to 1.5 equivalent, was observed, and the limit detection of A5 to Cu2+ was 0.11 μM. In addition, the Job's plot and mass spectrum showed that A5 complexed Cu2+ in a 1:1 manner. The apparent color change in the A5-Cu2+ complex under ultraviolet light at low molar concentrations revealed that A5 is a suitable probe for the detection of Cu2+. The biological test results show that the A5 probe has good biocompatibility and can be used for the cell imaging of Cu2+.
Keywords: bioimaging; copper ion; detection method; fluorescence probe; luminescence mechanism.