Lineage Tracing and Molecular Real-Time Imaging of Cancer Stem Cells

Xiaohua Jia; Guodong Shen; Jia Jia; Yan Zhang; Dan Zhang; Wanjun Li; Jianjun Zhang; Xinglu Huang; Jie Tian

doi:10.3390/bios12090703

Lineage Tracing and Molecular Real-Time Imaging of Cancer Stem Cells

Biosensors (Basel). 2022 Sep 1;12(9):703. doi: 10.3390/bios12090703.

Authors

Xiaohua Jia^{1

2}, Guodong Shen³, Jia Jia⁴, Yan Zhang², Dan Zhang⁵, Wanjun Li⁶, Jianjun Zhang⁷, Xinglu Huang⁸, Jie Tian^{1

9}

Affiliations

¹ Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
² Department of Ultrasound, General Hospital of People's Liberation Army, Beijing 100853, China.
³ Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
⁴ Fenyang College, Shanxi Medical University, Fenyang 032200, China.
⁵ Center of Biomedical Analysis, Tsinghua University, Beijing 100084, China.
⁶ Department of Pathology, Affiliated 3201 Hospital of Xi'an Jiaotong University, Hanzhong 723000, China.
⁷ Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.
⁸ Key Laboratory of Bioactive Materials for the Ministry of Education, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
⁹ Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, Beihang University, Beijing 100191, China.

Abstract

The cancer stem cells (CSC) are the roots of cancer. The CSC hypothesis may provide a model to explain the tumor cell heterogeneity. Understand the biological mechanism of CSC will help the early detection and cure of cancer. The discovery of the dynamic changes in CSC will be possible by the using of bio-engineering techniques-lineage tracing. However, it is difficult to obtain real-time, continuous, and dynamic live-imaging information using the traditional approaches that take snapshots of time points from different animals. The goal of molecular imaging is to monitor the in situ, continuous molecular changes of cells in vivo. Therefore, the most advanced bioengineering lineage tracing approach, while using a variety of molecular detection methods, will maximize the presentation of CSC. In this review, we first introduce the method of lineage tracing, and then introduce the various components of molecular images to dynamic detect the CSC. Finally, we analyze the current situation and look forward the future of CSC detection.

Keywords: cancer stem cells; lineage tracing; molecular imaging.

Publication types

Review

MeSH terms

Animals
Neoplasms* / diagnostic imaging
Neoplasms* / pathology
Neoplastic Stem Cells* / pathology

Grants and funding

This study was supported by the National Key Research and Development Program of China No. 2017YFA0205200, 2017YFA0700401, the National Natural Science Foundation of China under Grant No. U21A20386, 62027901, 81930053, 81227901, 31871111, 8190070112, the Chinese Academy of Sciences under Grant No. GJJSTD20170004 and QYZDJ-SSW-JSC005, the Key Research Program of Frontier Sciences No. QYZDY-SSW-SMC013, the Beijing-Tianjin-Hebei Basic Research Cooperation Special Program No. 19JCZDJC65300, the CAS Key Laboratory of Mental Health Grant No. KLMH2020K02, the Laboratory Innovation Fund of Tsinghua University No. 531001019, 100006029, and the Project of High-Level Talents Team Introduction in Zhuhai City, Zhuhai HLHPTP20170.