A High-Throughput Search for SFXN1 Physical Partners Led to the Identification of ATAD3, HSD10 and TIM50

Nesrine Tifoun; Mourad Bekhouche; José M De Las Heras; Arnaud Guillaume; Sylvina Bouleau; Isabelle Guénal; Bernard Mignotte; Nathalie Le Floch

doi:10.3390/biology11091298

A High-Throughput Search for SFXN1 Physical Partners Led to the Identification of ATAD3, HSD10 and TIM50

Biology (Basel). 2022 Aug 31;11(9):1298. doi: 10.3390/biology11091298.

Authors

Nesrine Tifoun¹, Mourad Bekhouche¹, José M De Las Heras¹, Arnaud Guillaume¹, Sylvina Bouleau¹, Isabelle Guénal¹, Bernard Mignotte^{1

2}, Nathalie Le Floch^{1

3}

Affiliations

¹ Laboratoire de Génétique et Biologie Cellulaire, UVSQ, Université Paris-Saclay, 78000 Versailles, France.
² Ecole Pratique des Hautes Etudes, PSL Research University, 75014 Paris, France.
³ IUT de Vélizy/Rambouillet, UVSQ, Université Paris-Saclay, 78120 Rambouillet, France.

Abstract

Sideroflexins (SFXN, SLC56) are a family of evolutionarily conserved mitochondrial carriers potentially involved in iron homeostasis. One member of the SFXN family is SFXN1, recently identified as a human mitochondrial serine transporter. However, little is known about the SFXN1 interactome, necessitating a high-throughput search to better characterize SFXN1 mitochondrial functions. Via co-immunoprecipitation followed by shotgun mass spectrometry (coIP-MS), we identified 96 putative SFXN1 interactors in the MCF7 human cell line. Our in silico analysis of the SFXN1 interactome highlights biological processes linked to mitochondrial organization, electron transport chains and transmembrane transport. Among the potential physical partners, ATAD3A and 17β-HSD10, two proteins associated with neurological disorders, were confirmed using different human cell lines. Nevertheless, further work will be needed to investigate the significance of these interactions.

Keywords: 17β-HSD10; ATAD3A; SFXN1; TIM50; mitochondria; sideroflexin.

Abstract

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