Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract

Fatimah A M Al-Zahrani; Salem S Salem; Huda A Al-Ghamdi; Laila M Nhari; Long Lin; Reda M El-Shishtawy

doi:10.3390/bioengineering9090452

Green Synthesis and Antibacterial Activity of Ag/Fe₂O₃ Nanocomposite Using Buddleja lindleyana Extract

Bioengineering (Basel). 2022 Sep 8;9(9):452. doi: 10.3390/bioengineering9090452.

Authors

Fatimah A M Al-Zahrani¹, Salem S Salem², Huda A Al-Ghamdi³, Laila M Nhari³, Long Lin⁴, Reda M El-Shishtawy^{5

6}

Affiliations

¹ Chemistry Department, Faculty of Science, King Khalid University, Abha 61413, Saudi Arabia.
² Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
³ Chemistry Department, Faculty of Science, University of Jeddah, Jeddah 21959, Saudi Arabia.
⁴ Colour Science, School of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK.
⁵ Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
⁶ Dyeing, Printing and Textile Auxiliaries Department, Institute of Textile Research and Technology, National Research Centre, 33 EL Buhouth St., Dokki, Giza 12622, Egypt.

Abstract

In the study reported in this manuscript, silver/iron oxide nanocomposites (Ag/Fe₂O₃) were phytosynthesized using the extract of Buddleja lindleyana via a green, economical and eco-friendly strategy. The biosynthesized Ag/Fe₂O₃ nanocomposites were characterized using UV-Vis spectrophotometry, FTIR, XRD, TEM, DLS and SEM-EDX analyses. The particulates showed a triangular and spherical morphology having sizes between 25 and 174 nm. FTIR studies on the nanoparticles showed functional groups corresponding to organic metabolites, which reduce and stabilize the Ag/Fe₂O₃ nanocomposite. The antimicrobial efficacy of the phytosynthesized Ag/Fe₂O₃ against bacterial pathogens was assessed. In addition, Ag/Fe₂O₃ exhibited broad spectrum activities against B. subtilis, S. aureus, E. coli, and P. aeruginosa with inhibition zones of 23.4 ± 0.75, 22.3 ± 0.57, 20.8 ± 1.6, and 19.5 ± 0.5 mm, respectively. The Ag/Fe₂O₃ composites obtained showed promising antibacterial action against human bacterial pathogens (S. aureus, E. coli, B. subtilis and P. aeruginosa), making them candidates for medical applications.

Keywords: Ag/Fe2O3; Buddleja lindleyana; antimicrobial activity; nanocomposite; phytosynthesis.

Grants and funding

RGP.1/252/43/Small Groups Project under grant number (RGP.1/252/43) Deanship of Scientific Research at King Khalid University