3D multiphoton lithography using biocompatible polymers with specific mechanical properties

Boris Buchroithner; Delara Hartmann; Sandra Mayr; Yoo Jin Oh; Dmitry Sivun; Andreas Karner; Bianca Buchegger; Thomas Griesser; Peter Hinterdorfer; Thomas A Klar; Jaroslaw Jacak

doi:10.1039/d0na00154f

3D multiphoton lithography using biocompatible polymers with specific mechanical properties

Nanoscale Adv. 2020 May 9;2(6):2422-2428. doi: 10.1039/d0na00154f. eCollection 2020 Jun 17.

Authors

Affiliations

¹ University of Applied Sciences Upper Austria, School of Medical Engineering and Applied Social Sciences Garnison Str. 21 4020 Linz Austria jaroslaw.jacak@fh-linz.at.
² Chair of Chemistry of Polymeric Materials, Montanuniversitaet Leoben Otto-Glöckel Str. 2 8700 Leoben Austria.
³ Institute of Biophysics, Johannes Kepler University Linz Gruberstr. 40 4020 Linz Austria.
⁴ Institute of Applied Physics and Linz Institute of Technology LIT, Johannes Kepler University Linz Altenberger Str. 69 4040 Linz Austria.

Abstract

The fabrication of two- and three-dimensional scaffolds mimicking the extracellular matrix and providing cell stimulation is of high importance in biology and material science. We show two new, biocompatible polymers, which can be 3D structured via multiphoton lithography, and determine their mechanical properties. Atomic force microscopy analysis of structures with sub-micron feature sizes reveals Young's modulus values in the 100 MPa range. Assessment of biocompatibility of the new resins was done by cultivating human umbilical vein endothelial cells on two-dimensionally structured substrates for four days. The cell density and presence of apoptotic cells has been quantified.