Genetic Loci in Phospholipase C-Like 1 (PLCL1) are Protective Factors for Allergic Rhinitis in Han Population of Northern Shaanxi, China

Wenxia Ruan; Rui Liu; Huimin Yang; Jiajia Ren; Yonglin Liu

doi:10.2147/JAA.S372314

Genetic Loci in Phospholipase C-Like 1 (PLCL1) are Protective Factors for Allergic Rhinitis in Han Population of Northern Shaanxi, China

J Asthma Allergy. 2022 Sep 14:15:1321-1335. doi: 10.2147/JAA.S372314. eCollection 2022.

Authors

Wenxia Ruan¹, Rui Liu², Huimin Yang¹, Jiajia Ren², Yonglin Liu²

Affiliations

¹ Clinical Laboratory, Shenmu Hospital, The Affiliated Shenmu Hospital of Northwest University, Shenmu, Shaanxi, 719300, People's Republic of China.
² Department of Science and Education, Shenmu Hospital, The Affiliated Shenmu Hospital of Northwest University, Shenmu, Shaanxi, 719300, People's Republic of China.

Abstract

Background: Allergic rhinitis (AR) is a common allergic disease in otolaryngology. Its pathogenesis is still unclear. PLC1 plays a key role in calcium homeostasis and immune response, which is potentially related to AR. We aimed to explore the association between PLCL1 genetic loci and susceptibility to AR.

Methods: We recruited 1975 volunteers to perform an association analysis through SNPStats online software. False-positive report probability (FPRP) analysis was used to detect whether the positive findings were worth noting. Linkage disequilibrium and haplotype analysis were completed through Haploview and SNPStats. The influence of SNP-SNP interaction on AR susceptibility was evaluated through multifactor dimensionality reduction (MDR).

Results: The results showed that four genetic loci in PLCL1 (rs2139049, rs212164068, rs2228135, and rs6738825) are associated with AR susceptibility under multiple genetic models. Allele "A" of PLCL1-rs2139049 (OR = 0.85, p = 0.031) or of -rs212164068 (OR = 0.85, p = 0.030), and allele "G" of PLCL1-rs6738825 (OR = 0.84, p = 0.022) are significantly associated with reduced AR risk. PLCL1-rs2228135 is associated with an increased risk of AR in males or participants older than 43 years of age. FPRP analysis showed that most of positive results are noteworthy findings. Three loci model composed of rs2139049, rs2164068, and rs2228135 is the best model for predicting AR risk (p = 0.0022). In addition, the haplotype "G_rs2139049A_rs6738825A_rs2164068A_rs2228135" (OR = 0.50, p = 0.033) can reduce the AR risk.

Conclusion: Allele "A" of PLCL1-rs2139049, allele "A" of -rs212164068, and allele "G" of PLCL1-rs6738825 are protective factors of AR in Han population from northern Shaanxi, China.

Keywords: PLCL1; allergic rhinitis; association analysis; genetic loci.

Grants and funding

This study was supported by the Natural Science Foundation of Shaanxi Province (2021SF-075), Science and Technology Plan, the Project of Yulin City (YF-2020-191) and Shenmu Municipal and the Government Scientific Research Project (2019) No. 5.