Dose-dependent Action of Zingiber officinale on Colonic Dysmotility and Ex Vivo Spontaneous Intestinal Contraction Modulation

Chaima Abidi; Kais Rtibi; Salima Boutahiri; Haifa Tounsi; Afifa Abdellaoui; Soumaya Wahabi; Bernard Gressier; Bruno Eto; Hichem Sebai

doi:10.1177/15593258221127556

Dose-dependent Action of Zingiber officinale on Colonic Dysmotility and Ex Vivo Spontaneous Intestinal Contraction Modulation

Dose Response. 2022 Sep 15;20(3):15593258221127556. doi: 10.1177/15593258221127556. eCollection 2022 Jul-Sep.

Authors

Chaima Abidi¹, Kais Rtibi¹, Salima Boutahiri², Haifa Tounsi³, Afifa Abdellaoui³, Soumaya Wahabi¹, Bernard Gressier⁴, Bruno Eto⁵, Hichem Sebai¹

Affiliations

¹ Laboratory of Functional Physiology and Valorization of Bio-Ressources-Higher Institute of Biotechnology of Beja, University of Jendouba, Beja, Tunisia.
² Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes, Morocco.
³ Laboratory of Human and Experimental Pathological Anatomy, Pasteur Institute of Tunis, Beja, Tunisia.
⁴ Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmacy, University of Lille, Lille, France.
⁵ Laboratories TBC, Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmacy, University of Lille, Lille, France.

Abstract

Ginger (Zingiber officinale) rhizomes are commonly used in foods and employed for many ailments including gastrointestinal disorders. Our main objective was to evaluate the effect of Zingiber officinale aqueous extract (ZOAE) on gastrointestinal (GI) physiological motility and colonic dysmotility. Thereby, Wistar rats were given loperamide (LP, 3 mg/kg, b.w.) and ZOAE (75, 150, and 300 mg/kg, b.w.) or yohimbine (YOH, 2 mg/kg, b.w.). ZOAE-action on intestinal secretion was assessed using Ussing chamber technique and intestinal motility with isometric transducer. GI-transit (GIT) and gastric emptying (GE) were evaluated with the charcoal meal test and the red phenol methods. ZOAE-bioactive components were analyzed by liquid chromatography-high resolution electrospray ionization mass spectrometry (LC-HRESIMS). Constipation was induced with LP and the different indicators such as stool composition, GIT, oxidative stress biological parameters, and colonic mucosa histological alteration were performed. Anti-constipation effect of ZOAE was confirmed on stool composition, GIT (53.42% to 85.57%), GE (55.47% to 98.88%), and re-established oxidative balance. ZOAE induces an amplitude increase of spontaneous intestinal contraction with EC50 of 10.52 μg/mL. No effect of ZOAE was observed on electrogenic transport of intestinal fluid. These findings suggest that ZOAE-bioactive candidates might exert an anti-constipation action and spontaneous intestinal contraction modulation.

Keywords: Zingiber officinale; antioxidant activity; colonic dysmotility; gastro-intestinal transit; ginger rhizomes; intestinal contraction; rats; spontaneous intestinal contraction.