Sperm cryopreservation is vital in combating the human infertility, but regrettably, the toxicity of cryoprotectants and the occurrence of intracellular icing, osmotic shocks or shrinkage of the cells below a given threshold volume greatly affects the success rate of this technique. Using the virtue of nanotechnologies and depositing water-repellent soot nanoparticles on the inner walls of cryovials may outline new directions in the development of cryobiology, but doubts related to the soot's venomosity question its practical implementability. The scientific content of this article eliminates the existing apprehensions by analyzing the cytotoxicity of three types of rapeseed oil soot, differing in morphology, surface chemistry and zeta potential, towards human spermatozoa. Upon intermittent evaluations of the sperm motility within 270 min of incubation in vials comprising carbon nanoparticles, we reveal that this soot category is non-cytotoxic or at worst, faintly toxic to the gametes provided by twenty individuals. Enhanced progressive sperm motility is observed at ∼50-60% of patients following the soot treatments, which is attributed to electrostatic repulsions and biochemical alterations in the seminal plasma. These fascinating results open new horizons for incorporation of the rapeseed oil soot as a tool for functional preparation and activation of human spermatozoa preceding in vitro fertilization.
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