Comparative transcriptome reveals the effect of IFITM1 on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks

Suyun Liang; Xiaoyang Hu; Zhanbao Guo; Dawei Luo; Jing Tang; Zhanqing Ji; Ming Xie; Shuisheng Hou

doi:10.1016/j.virusres.2022.198930

Comparative transcriptome reveals the effect of IFITM1 on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks

Virus Res. 2022 Dec:322:198930. doi: 10.1016/j.virusres.2022.198930. Epub 2022 Sep 18.

Authors

Suyun Liang¹, Xiaoyang Hu², Zhanbao Guo¹, Dawei Luo¹, Jing Tang¹, Zhanqing Ji¹, Ming Xie¹, Shuisheng Hou³

Affiliations

¹ Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian district, Beijing 100193, China.
² College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
³ Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian district, Beijing 100193, China. Electronic address: houss@263.net.

PMID: 36130655
DOI: 10.1016/j.virusres.2022.198930

Abstract

Background: Duck viral hepatitis (DVH) has a significant economic impact on duck industry, and duck hepatitis A virus genotype 3 (DHAV-3) is the most prevalent pathogen of DVH in Asian duck industry. The detailed study connecting differentially expressed genes (DEGs) and the differential resistance to DHAV-3 have not been accurately described, although a large numbers of DEGs have been identified by transcriptomic studies.

Results: Here, a resistant Pekin duck line (Z8R) and a susceptible Pekin duck line (Z8S) as models, high mortality and dramatically increased aspartate aminotransferase (AST), alanine aminotransferase (ALT) and the expression of immune-related genes of Z8S group were shown to be noticeable signs of cases caused by DHAV-3 infection. Compared with the control (Con) group, 1117 down-regulated DEGs and 612 up-regulated DEGs were found in the Z8S group and 37 down-regulated DEGs and 82 up-regulated DEGs were found in the Z8R group. Ultimately, the expression patterns of 10 DEGs were found to be diametrically opposite in Z8R and Z8S group. Functional analysis revealed that IFITM1 was associated with cell growth suppression, which was considered a key candidate gene. Results of flow cytometry showed that the conserved regions of IFITM1 (213-317 bp) could affected the cell cycle of duck embryo fibroblast (DEF) cells after infection with DHAV-3. Transcriptome and western blot analysis suggested that the CCND1, CCNE1 and CDK6 were significantly up-regulated in susceptible ducks by comparing with Con group.

Conclusions: The hepatic injury and pathogenic outcomes caused by DHAV-3 infection were more severe in Z8S group compared to Z8R. Results of transcriptomics analysis and flow cytometry suggested that DHAV-3 infection can induce cell cycle changes that may be associated with IFITM1 expression level. These data will greatly enhance our understanding of the pathogenesis of DHAV-3 infection in ducklings and have implications for development of resistance breeding.

Keywords: DHAV-3; Gene expression; IFITM1; Pekin duck; Transcriptome analysis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ducks
Genotype
Hepatitis Virus, Duck* / genetics
Hepatitis, Viral, Animal*
Picornaviridae Infections* / genetics
Picornaviridae Infections* / pathology
Picornaviridae Infections* / veterinary
Poultry Diseases*
Transcriptome