Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii

Diego Rivera Gelsinger; Jocelyne DiRuggiero

doi:10.1007/978-1-0716-2445-6_15

Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii

Methods Mol Biol. 2022:2522:243-254. doi: 10.1007/978-1-0716-2445-6_15.

Authors

Diego Rivera Gelsinger¹, Jocelyne DiRuggiero²

Affiliations

¹ Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
² Department of Biology and Department of Earth and Planetary Sciences, Johns Hopkins University, Baltimore, MD, USA. jdiruggiero@jhu.edu.

PMID: 36125754
DOI: 10.1007/978-1-0716-2445-6_15

Abstract

Posttranscriptional regulation actuated by small RNAs (sRNAs) plays essential roles in a wide variety of cellular processes, especially in stress responses and environmental signaling. Hundreds of sRNAs have recently been discovered in archaea using genome-wide approaches but the molecular mechanisms of only a few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries using size-selected total RNA in the model archaeon, Haloferax volcanii , to provide a tool to further characterize sRNAs in archaea.

Keywords: Haloarchaea; Noncoding RNA; Sequencing libraries; Small RNA; Transcription.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Gene Library
Haloferax volcanii* / genetics
RNA, Archaeal / genetics
RNA, Small Untranslated* / genetics
Sequence Analysis, RNA

Substances

RNA, Archaeal
RNA, Small Untranslated