Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.
Keywords: Bile canaliculus; Hepatocyte; Hepatocyte polarity; Induced pluripotent stem cell; Inherited liver disease.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.