Cross-Talk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana

Liyun Song; Huaixu Zhan; Yujie Wang; Zhonglong Lin; Bin Li; Lili Shen; Yubing Jiao; Ying Li; Fenglong Wang; Jinguang Yang

doi:10.1021/acsomega.2c03917

Cross-Talk of Protein Expression and Lysine Acetylation in Response to TMV Infection in Nicotiana benthamiana

ACS Omega. 2022 Sep 1;7(36):32496-32511. doi: 10.1021/acsomega.2c03917. eCollection 2022 Sep 13.

Authors

Liyun Song¹, Huaixu Zhan^{1

2}, Yujie Wang³, Zhonglong Lin⁴, Bin Li⁵, Lili Shen¹, Yubing Jiao¹, Ying Li¹, Fenglong Wang¹, Jinguang Yang¹

Affiliations

¹ Key Laboratory of Tobacco Pest Monitoring, Controlling & Integrated Management, Tobacco Research Institute of the Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
² Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
³ Luoyang Branch of Henan Tobacco Company, Luoyang 471000, China.
⁴ Yunnan Tobacco Company of the China National Tobacco Corporation, Kunming 650011, China.
⁵ Sichuan Tobacco Company, Chengdu 610017, China.

Abstract

Lysine acetylation (K^ac), a reversible PTM, plays an essential role in various biological processes, including those involving metabolic pathways, pathogen resistance, and transcription, in both prokaryotes and eukaryotes. TMV, the major factor that causes the poor quality of Solanaceae crops worldwide, directly alters many metabolic processes in tobacco. However, the extent and function of K^ac during TMV infection have not been determined. The validation test to detect K^ac level and viral expression after TMV infection and Nicotinamide (NAM) treatment clarified that acetylation was involved in TMV infection. Furthermore, we comprehensively analyzed the changes in the proteome and acetylome of TMV-infected tobacco (Nicotiana benthamiana) seedlings via LC-MS/MS in conjunction with highly sensitive immune-affinity purification. In total, 2082 lysine-acetylated sites on 1319 proteins differentially expressed in response to TMV infection were identified. Extensive bioinformatic studies disclosed changes in acetylation of proteins engaged in cellular metabolism and biological processes. The vital influence of K^ac in fatty acid degradation and alpha-linolenic acid metabolism was also revealed in TMV-infected seedlings. This study first revealed K^ac information in N. benthamiana under TMV infection and expanded upon the existing landscape of acetylation in pathogen infection.