Role of semi-purified andrographolide from Andrographis paniculata extract as nano-phytovesicular carrier for enhancing oral absorption and hypoglycemic activity

Vinod Kumar Verma; Md Kamaruz Zaman; Shekhar Verma; Santosh Kumar Verma; Khomendra Kumar Sarwa

doi:10.1016/j.chmed.2019.12.004

Role of semi-purified andrographolide from Andrographis paniculata extract as nano-phytovesicular carrier for enhancing oral absorption and hypoglycemic activity

Chin Herb Med. 2019 Dec 25;12(2):142-155. doi: 10.1016/j.chmed.2019.12.004. eCollection 2020 Apr.

Authors

Vinod Kumar Verma^{1

2}, Md Kamaruz Zaman¹, Shekhar Verma³, Santosh Kumar Verma^{4

5}, Khomendra Kumar Sarwa⁶

Affiliations

¹ Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India.
² State Food and Drug Testing Laboratory, Raipur 492001, India.
³ University College of Pharmacy, Pt. Deendayal Upadhyay Health, Science and Aayush University Raipur-492001, Chhattisgarh, India.
⁴ School of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, China.
⁵ Shaanxi Key Laboratory of Low Metamorphic Coal Clean Utilization, Yulin University, Yulin 719000, China.
⁶ Department of Pharmacy, Government Girl's Polytechnic, Raipur 492001, India.

Abstract

Objective: Andrographis paniculata is a well-known medicinal plant in Southeast Asia, India and China. The plant contains andrographolide (AN), a very important phytochemical used in various health problems. However, AN is low in oral absorption bioavailability of AN due to the rapid clearance and high protein binding capacity.

Methods: The present study was aimed to develop a nano-phytovesicular formulation of semi-purified AN extracts from a naturally occurring phospholipid (soya phosphatidylcholine) in order to increase the oral absorption and antihyperglycemic activity in rats.

Results: The nano-phyto vesicle of semi-purified AN extracts equivalent to 25 mg /kg AN significantly protected the hyperglycemic condition of rats. The in vitro and in vivo experiments results proved that the nano- phytovesicular system of plant extracts containing AN produced better oral absorption, bioavailability and improved antihyperglycemic activity compared with that of free AN at dose of 50 mg/kg.

Conclusion: Hence, the prepared semi-purified extract nano-phytovesicular system is helpful in solving the problem of rapid clearance of AN.

Keywords: Andrographis paniculata (Burm. f.) Nees; andrographolide; hypoglycemic activity; liquid dispersion; nano-phyto vesicle; soya phosphatidylcholine.