Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations

Li Xiaoshuai; Wang Qiushi; Wang Rui

doi:10.3389/fbioe.2022.932936

Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations

Front Bioeng Biotechnol. 2022 Sep 2:10:932936. doi: 10.3389/fbioe.2022.932936. eCollection 2022.

Authors

Li Xiaoshuai¹, Wang Qiushi¹, Wang Rui²

Affiliations

¹ Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, China.
² Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, National Health Commission of China and Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang, China.

Abstract

In the past 10 years, gene-editing and organoid culture have completely changed the process of biology. Congenital nervous system malformations are difficult to study due to their polygenic pathogenicity, the complexity of cellular and neural regions of the brain, and the dysregulation of specific neurodevelopmental processes in humans. Therefore, the combined application of CRISPR-Cas9 in organoid models may provide a technical platform for studying organ development and congenital diseases. Here, we first summarize the occurrence of congenital neurological malformations and discuss the different modeling methods of congenital nervous system malformations. After that, it focuses on using organoid to model congenital nervous system malformations. Then we summarized the application of CRISPR-Cas9 in the organoid platform to study the pathogenesis and treatment strategies of congenital nervous system malformations and finally looked forward to the future.

Keywords: 3D; CRISPR-Cas9; central nervous system; congenital nervous system malformation; organoid.

Publication types

Review