Differences, links, and roles of microbial and stoichiometric factors in microplastic distribution: A case study of five typical rice cropping regions in China

Yao Yao; Lili Wang; Lingxuan Gong; Gang Li; Weiming Xiu; Xiaomei Yang; Bingchang Tan; Jianning Zhao; Guilong Zhang

doi:10.3389/fmicb.2022.985239

Differences, links, and roles of microbial and stoichiometric factors in microplastic distribution: A case study of five typical rice cropping regions in China

Front Microbiol. 2022 Sep 2:13:985239. doi: 10.3389/fmicb.2022.985239. eCollection 2022.

Authors

Yao Yao¹, Lili Wang¹, Lingxuan Gong¹, Gang Li¹, Weiming Xiu¹, Xiaomei Yang^{2

3}, Bingchang Tan¹, Jianning Zhao¹, Guilong Zhang¹

Affiliations

¹ Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China.
² College of Natural Resources and Environment, Northwest A&F University, Yangling, China.
³ Soil Physics and Land Management Group, Wageningen University & Research, Wageningen, Netherlands.

Abstract

Microplastics (MPs), as new pollutants in agroecosystems, have already attracted widespread attention from scientists. However, our understanding of MP geographic distribution and its influencing factors across spatial scales remains poor. Here, a regional-scale field investigation was conducted to assess the distribution characteristic of MPs in five major rice-growing regions of China, and we explored the roles of biological and abiotic factors, especially stoichiometry and microbial influences on MP distribution. MPs were observed in all sampling sites, averaging 6,390 ± 2,031 items⋅kg^-1. Sizes less than 0.5 mm and black and transparent MPs dominated. Fiber, classified as one of the MP shapes, occurred most frequently. MP community analysis, firstly used in paddy soil, revealed more black MPs abundance in Henan (HE), more rayon, blue, and other colors MPs in Hunan (HN), more transparent MPs in Tianjing (TJ), and more PE MPs in Heilongjiang (DB). Higher MP community diversity was found in most south paddy soils of this study, due to a broader range of sources. C/N showed a positive relationship with pellet-shaped MP abundance and MPs of size between 2 and 5 mm (P < 0.05). Chao1 index of soil microbial communities was positively correlated with the MP abundance, MPs of size less than 0.5 mm, and fiber abundance. The minimum temperature was positively correlated with MP abundance (P < 0.05), implying the potential effects of the freeze-thaw process might exist. The regression analysis highlighted the important role of population quantity in determining MP abundance (R = 0.421, P = 0.02). This study confirmed the wide distribution of MPs in different soil depths of paddy lands in China and demonstrated that its distribution was influenced by population quantity and environmental variables, such as microbiology. These findings could provide a basis for the toxicological behavior of MPs and the potential risk to human health.

Keywords: bacterial diversity; microbiology; microplastics; paddy soils; stoichiometric ratio.