Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis

Jayne Culley; Peter W Nagle; John C Dawson; Neil O Carragher

doi:10.1016/j.slasd.2022.09.002

Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis

SLAS Discov. 2023 Mar;28(2):13-19. doi: 10.1016/j.slasd.2022.09.002. Epub 2022 Sep 16.

Authors

Jayne Culley¹, Peter W Nagle², John C Dawson¹, Neil O Carragher³

Affiliations

¹ Cancer Research UK Edinburgh Centre, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, EH4 2XR, UK.
² Cancer Research UK Edinburgh Centre, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, EH4 2XR, UK; MRC Centre for Reproductive Health, University of Edinburgh, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, Scotland, EH16 4TJ, UK.
³ Cancer Research UK Edinburgh Centre, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, EH4 2XR, UK. Electronic address: n.carragher@ed.ac.uk.

PMID: 36116760
DOI: 10.1016/j.slasd.2022.09.002

Abstract

Three dimensional models of cell culture enables researchers to recreate aspects of tumour biology not replicated by traditional two dimensional techniques. Here we describe a protocol to enable automated high throughput phenotypic profiling across panels of patient derived glioma stem cell spheroid models. We demonstrate the use of both live/dead cell end-points and monitor the dynamic changes in the cell cycle using cell lines expressing the FUCCI cell cycle reporter. Together, these assays provide additional insight into the mechanism of action of compound treatments over traditional cell viability assay endpoints.

Keywords: 3-dimensional; Cell cycle; Drug discovery; Glioblastoma; Spheroid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods
Cell Line
Glioma* / genetics
Humans
Spheroids, Cellular*
Stem Cells

Abstract

Publication types

MeSH terms

Grants and funding