Lipid profiling analyses from mouse models and human infants

Laurentya Olga; Ivana Bobeldijk-Pastorova; Richard C Bas; Florine Seidel; Stuart G Snowden; Samuel Furse; Ken K Ong; Robert Kleemann; Albert Koulman

doi:10.1016/j.xpro.2022.101679

Lipid profiling analyses from mouse models and human infants

STAR Protoc. 2022 Dec 16;3(4):101679. doi: 10.1016/j.xpro.2022.101679. Epub 2022 Sep 15.

Authors

Laurentya Olga¹, Ivana Bobeldijk-Pastorova², Richard C Bas³, Florine Seidel³, Stuart G Snowden⁴, Samuel Furse⁴, Ken K Ong⁵, Robert Kleemann⁶, Albert Koulman⁷

Affiliations

¹ Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK.
² Department of Metabolic Health Research, The Netherlands Organization for Applied Scientific Research (TNO), 2333 CK Leiden, the Netherlands.
³ DUCARES B.V. | trading as TRISKELION, Reactorweg 47-A, 3542 AD Utrecht, the Netherlands.
⁴ Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK.
⁵ Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK.
⁶ Department of Vascular Surgery, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands.
⁷ Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK. Electronic address: ak675@medschl.cam.ac.uk.

Abstract

This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort. For complete details on the use and execution of this protocol, please refer to Olga et al. (2021).

Keywords: Clinical protocol; Health sciences; Mass spectrometry; Metabolism; Metabolomics; Systems biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Humans
Infant
Lipidomics*
Lipids*
Mice

Substances

Lipids

Abstract

Publication types

MeSH terms

Substances

Grants and funding