Stabilization of guinea pig transglutaminase 2 solutions

Thomas M Jeitner; James M Kelly

doi:10.1016/j.ab.2022.114885

Stabilization of guinea pig transglutaminase 2 solutions

Anal Biochem. 2022 Nov 15:657:114885. doi: 10.1016/j.ab.2022.114885. Epub 2022 Sep 13.

Authors

Thomas M Jeitner¹, James M Kelly²

Affiliations

¹ Department of Radiology, Weill Cornell Medicine, United States. Electronic address: tmj4001@med.cornell.edu.
² Department of Radiology, Weill Cornell Medicine, United States.

PMID: 36113550
DOI: 10.1016/j.ab.2022.114885

Abstract

Mammalian transglutaminase 2 exhibits poor long-term stability in solution. Reconstituting lyophilized transglutaminase 2 in solutions containing dithiothreitol and EDTA alone and together with glycerol stabilizes the activity of this enzyme for several weeks.

MeSH terms

Animals
Dithiothreitol
Edetic Acid
Glycerol
Guinea Pigs
Liver
Mammals
Protein Glutamine gamma Glutamyltransferase 2*
Transglutaminases*

Substances

Edetic Acid
Protein Glutamine gamma Glutamyltransferase 2
Transglutaminases
Glycerol
Dithiothreitol