Intermittent voltage supply has been reported to improve the performance of electro-assisted anaerobic digestion but has not been well understood. In this study, an intermittent voltage of 0.6 V (1 day on-1 day off) was applied in an electro-assisted anaerobic digester to explore its effects. Compared to those without the voltage, the methane yield increased nearly by 20.0%, and organic decomposition increased by 9.5% with the intermittent voltage, which was similar to those with the continuous voltage. The amide groups of the sludge protein after the electro-treatment were polarized to enhance electron transfer and electron storage of protein-like substances of the sludge. Although the voltage was supplied intermittently, the increased conductivity and capacitance of the sludge and EPS could effectively transport electrons between exoelectrogens and electrotrophs (such as Firmicutes and Methanothrix) to promote the anaerobic digestion. This study explained the essence of electrochemical enhancement of anaerobic digestion from the perspective of molecular structure, that is, the polarization of functional groups by voltage could improve the sludge electro-activity to maintain effective interspecies electron transfer in the periodic voltage supply.
Keywords: Anaerobic digestion; Extracellular electron transfer; Intermittent power supply; Interspecies electron transfer.
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