Rapid genotyping of targeted viral samples using Illumina short-read sequencing data

PLoS One. 2022 Sep 16;17(9):e0274414. doi: 10.1371/journal.pone.0274414. eCollection 2022.

Abstract

The most important information about microorganisms might be their accurate genome sequence. Using current Next Generation Sequencing methods, sequencing data can be generated at an unprecedented pace. However, we still lack tools for the automated and accurate reference-based genotyping of viral sequencing reads. This paper presents our pipeline designed to reconstruct the dominant consensus genome of viral samples and analyze their within-host variability. We benchmarked our approach on numerous datasets and showed that the consensus genome of samples could be obtained reliably without further manual data curation. Our pipeline can be a valuable tool for fast identifying viral samples. The pipeline is publicly available on the project's GitHub page (https://github.com/laczkol/QVG).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genome
  • Genotype
  • High-Throughput Nucleotide Sequencing* / methods
  • Software*

Grants and funding

Financial support was achieved by the Economic Development and Innovation Operational Programme (GINOP-2.3.4-15-2020-00008) in the frame of the Complex Health Multidisciplinary Competence Center at the University of Debrecen. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.