Evaluation of the In-Vitro Cytotoxicity of Heat Cure Denture Base Resin Modified with Recycled PMMA-Based Denture Base Resin

Divya Krishnamoorthi; Suma Karthigeyan; Seyed Asharaf Ali; Sasikala Rajajayam; Ramya Gajendran; Mithra Rajendran

doi:10.4103/jpbs.jpbs_890_21

Evaluation of the In-Vitro Cytotoxicity of Heat Cure Denture Base Resin Modified with Recycled PMMA-Based Denture Base Resin

J Pharm Bioallied Sci. 2022 Jul;14(Suppl 1):S719-S725. doi: 10.4103/jpbs.jpbs_890_21. Epub 2022 Jul 13.

Authors

Divya Krishnamoorthi¹, Suma Karthigeyan², Seyed Asharaf Ali³, Sasikala Rajajayam¹, Ramya Gajendran⁴, Mithra Rajendran¹

Affiliations

¹ Department of Prosthodontics and Crown and Bridge, Vinayaka Mission's Sankarachariyar Dental College, A Constituent College of Vinayaka Mission's Research Foundation (Deemed to be University), Ariyanoor, Tamil Nadu, India.
² Department of Prosthodontics, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
³ Department of Prosthodontics and Crown & Bridge, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
⁴ DRN Skin and Dental Care Clinic, Anna Nagar, Chennai, Tamil Nadu, India.

Abstract

Aim: The aim of the present study was to evaluate the in-vitro cytotoxicity of heat-cure denture base resin (PMMA) modified with recycled denture base resin at 10%, 20%, 30%, 40%, and 50% (w/w) concentration.

Materials and methods: A total of 30 disk-shaped specimens were prepared and divided into six groups (n = 5). The Control group (R₀) consisted of unmodified processed denture base resin, the experimental group consisted of denture base resin processed with substitution of 10% 20%, 30%, 40%, and 50% (w/w) of recycled denture base resin (R₁₀, R_20, R_30, R₄₀, and R₅₀). Eluates were prepared using five sterile specimens of each group. The mouse fibroblast cell line (L929) was seeded in a 96-well cell plate system at a concentration of 1 × 10⁴ cells/well in the DMEM medium with 1× antibiotic and antimycotic solution and 10% fetal bovine serum at 37°C with 5% CO₂ and incubated in a CO₂ incubator for 48 h. MTT assay was applied and the absorbance was measured at 570 nm using a microplate reader to assess the in-vitro cytotoxicity. One-way analysis of variance (ANOVA) along with post hoc Scheffe test was used to statistically compare the mean optical density (OD) values and cell survival/viability % amongst the groups.

Results: No statistically significant difference was observed in the mean and standard deviation of the optical density and cell viability % of the test groups that were compared.

Conclusion: Modification of denture base resin using recycled PMMA does not have a cytotoxic effect on the mouse fibroblast cell line L929.

Keywords: Cell survival; cytotoxicity; polymer; recycling; reuse.