Effect of piperine on the mitigation of obesity associated with gut microbiota alteration

Jianlin He; Qingqing Le; Yufeng Wei; Longhe Yang; Bing Cai; Yuansen Liu; Bihong Hong

doi:10.1016/j.crfs.2022.08.018

Effect of piperine on the mitigation of obesity associated with gut microbiota alteration

Curr Res Food Sci. 2022 Sep 2:5:1422-1432. doi: 10.1016/j.crfs.2022.08.018. eCollection 2022.

Authors

Jianlin He^{1

2

3}, Qingqing Le^{1

2

3}, Yufeng Wei⁴, Longhe Yang^{1

2

3}, Bing Cai^{1

2

3}, Yuansen Liu^{1

2

3}, Bihong Hong^{1

2

3}

Affiliations

¹ Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.
² Technology Innovation Center for Exploitation of Marine Biological Resources, Ministry of Natural Resources, Xiamen, 361005, China.
³ Fujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Pingtan, 350400, China.
⁴ Department of Chemistry and Biochemistry, New Jersey City University, Jersey City, NJ, 07305, USA.

Abstract

An obese mouse model induced by high-fat diet (HFD) feeding was used to reveal the role of piperine in modulating gut microbiota (GM). Piperine was administrated at 20 and 40 mg/kg body weight every day. As a result, piperine at 40 mg/kg significantly decreased body weight, liver weight, perirenal fat weight, and lowered serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose levels in HFD-fed mice. Additionally, piperine significantly attenuated fatty liver and modulated hepatic mRNA expressions of SREBP-1c, SREBP2, and HMGCR. In perirenal fat, FAS, C/EBPα, MCP1, and IL-6 expressions were significantly downregulated by piperine. 16S rRNA sequencing revealed that piperine elevated GM diversity. The relative abundance of Muribaculaceae and Ruminococcaceae were significantly elevated, while Dubosiella and Enterorhabdus genera were suppressed by piperine. The Pearson correlation analysis showed that the altered phylotypes were highly correlated with obesity phenotypes. These findings suggest that piperine modulates energy homeostasis and inflammation to alleviate obesity associated with GM regulation.

Keywords: Fatty liver; Muribaculaceae; Obesity; Piperine; Ruminococcaceae.