The Edible Seaweed Gelidium amansii Promotes Structural Plasticity of Hippocampal Neurons and Improves Scopolamine-Induced Learning and Memory Impairment in Mice

Md Mohibbullah; Michael Yordan Pringgo Wicaksono; Md Abdul Hannan; Raju Dash; Maria Dyah Nur Meinita; Jae-Suk Choi; Yong-Ki Hong; Il Soo Moon

doi:10.2174/1871527321666220909142158

The Edible Seaweed Gelidium amansii Promotes Structural Plasticity of Hippocampal Neurons and Improves Scopolamine-Induced Learning and Memory Impairment in Mice

CNS Neurol Disord Drug Targets. 2022 Sep 9. doi: 10.2174/1871527321666220909142158. Online ahead of print.

Authors

Md Mohibbullah^{1

2

3

4}, Michael Yordan Pringgo Wicaksono⁴, Md Abdul Hannan^{5

6}, Raju Dash⁷, Maria Dyah Nur Meinita⁸, Jae-Suk Choi^{2

3}, Yong-Ki Hong⁴, Il Soo Moon⁷

Affiliations

¹ Department of Fishing and Post Harvest Technology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh.
² Seafood Research Center, Silla University, #605, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Korea.
³ Department of Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, Busan 46958, Korea.
⁴ Department of Biotechnology, Pukyong National University, Namku, Busan 608-737, Korea.
⁵ Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
⁶ Department of Anatomy, College of Korean Medicine, Dongguk University, 38066, Korea.
⁷ Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju, Gyeongbuk 38066, Korea.
⁸ Faculty of Fisheries and Marine Science, Center for Maritime Biosciences Studies, Jenderal Soedirman University, Purwokerto 53123, Indonesia.

PMID: 36093815
DOI: 10.2174/1871527321666220909142158

Abstract

Background: Gelidium amansii has been gaining profound interest in East Asian countries due to its enormous commercial value for agar production and its extensive pharmacological properties. Previous studies have shown that the ethanol extract of Gelidium amansii (GAE) has promising neurotrophic effects in in vitro conditions.

Objectives: The present study aimed at investigating the protective effects of GAE against scopolamine-induced cognitive deficits and its modulatory effects on hippocampal plasticity in mice.

Methods: For memory-related behavioral studies, the passive avoidance test and radial arm maze paradigm were conducted. The brain slices of the hippocampus CA1 neurons of experimental mice were then prepared to perform Golgi staining for analyzing spine density and its characteristic shape, and immunohistochemistry for assessing the expression of different pre- and postsynaptic proteins.

Results: Following oral administration of GAE (0.5 mg/g body weight), mice with memory deficits exhibited a significant increase in the latency time on the passive avoidance test and a decrease in the number of working and reference memory errors and latency time on the radial arm maze test. Microscopic observations of Golgi-impregnated tissue sections and immunohistochemistry of hippocampal slices showed that neurons from GAE-treated mice displayed higher spine density and spine dynamics, increased synaptic contact, and the recruitment of memory-associated proteins such as N-methyl-D-aspartate receptors (NR2A and NR2B) and postsynaptic density-95 (PSD-95) when compared with the control group.

Conclusion: With these memory-protective functions and a modulatory role in underlying memory-related events, GAE could be a potential functional food and a promising source of pharmacological agents for the prevention and treatment of memory-related brain disorders.

Keywords: Gelidium amansii; GluN2A; PSD-95.; cognition; spine dynamics; synaptogenesis.