Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids

Sergio Pedraza-Arevalo; Ana-Maria Cujba; Mario Enrique Alvarez-Fallas; Rocio Sancho

doi:10.1016/j.xpro.2022.101656

Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids

STAR Protoc. 2022 Aug 30;3(3):101656. doi: 10.1016/j.xpro.2022.101656. eCollection 2022 Sep 16.

Authors

Sergio Pedraza-Arevalo¹, Ana-Maria Cujba¹, Mario Enrique Alvarez-Fallas¹, Rocio Sancho²

Affiliations

¹ Center for Gene Therapy and Regenerative Medicine, Kings College London, London, SE1 9RT, UK.
² Center for Gene Therapy and Regenerative Medicine, Kings College London, London, SE1 9RT, UK; Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany. Electronic address: rocio.sancho@kcl.ac.uk.

Abstract

Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).

Keywords: Cell differentiation; Developmental biology; Metabolism; Organoids; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Humans
Induced Pluripotent Stem Cells*
Organoids
Pluripotent Stem Cells*

Abstract

Publication types

MeSH terms

Grants and funding