The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells

Ryoma Okawa; Koushirou Sogawa; Motoko Shiozaki; Kenji Yachiku; Takanori Miura; Takashi Shibata; Sachiko Ezoe

doi:10.1016/j.reth.2022.07.014

The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells

Regen Ther. 2022 Aug 23:21:250-257. doi: 10.1016/j.reth.2022.07.014. eCollection 2022 Dec.

Authors

Ryoma Okawa¹, Koushirou Sogawa², Motoko Shiozaki¹, Kenji Yachiku¹, Takanori Miura¹, Takashi Shibata³, Sachiko Ezoe^{1

4}

Affiliations

¹ Department of Space Infection Control, Graduate School of Medicine/Faculty of Medicine, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
² Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Shonan University of Medical Sciences, 16-10, Kamishinano, Totsuka, Yokohama, Kanagawa 244-0806, Japan.
³ Strategic Global Partnership Cross-Innovation Initiative, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
⁴ Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Abstract

Background: Recently, various regenerative therapies have been developed based on induced pluripotent stem (iPS) cells. However, hygienic control strategies have not been established at the manufacturing facilities. We aimed to evaluate the safety and effects of continuous exposure to low-dose chlorine dioxide (ClO₂) gas on cell fates, and to determine the optimum dose for safe usage of this disinfectant.

Methods: We cultured an iPS cell line in the absence or presence of various doses of ClO₂ gas. We evaluated cell proliferation, cell death, the maintenance of undifferentiated state, and cell senescence.

Results: We found that iPS cell proliferation was not affected by 0.05 or 0.1 ppmv ClO₂ gas in the atmosphere. Although 0.1 ppmv ClO₂ slightly affected apoptosis, it was not a significant effect. Moreover, neither at 0.05 nor 0.1 ppmv ClO₂ gas significantly affected the characteristics of iPS cells.

Discussion and conclusion: Continuous exposure to 0.05 or 0.1 ppmv ClO₂ gas did not affect the fate of iPS cells. These results may contribute to the development of new strategies for hygiene control in cell processing facilities.

Keywords: Cell fate; Disinfectant; Low dose ClO2 gas; Stem cells; iPS cells.