Understanding physiological mechanisms of variation in grain filling of maize under high planting density and varying nitrogen applicate rate

Hong Ren; Ming Zhao; Baoyuan Zhou; Wenbin Zhou; Kemin Li; Hua Qi; Ying Jiang; Congfeng Li

doi:10.3389/fnut.2022.998946

Understanding physiological mechanisms of variation in grain filling of maize under high planting density and varying nitrogen applicate rate

Front Nutr. 2022 Aug 24:9:998946. doi: 10.3389/fnut.2022.998946. eCollection 2022.

Authors

Hong Ren^{1

2}, Ming Zhao¹, Baoyuan Zhou¹, Wenbin Zhou¹, Kemin Li¹, Hua Qi², Ying Jiang², Congfeng Li¹

Affiliations

¹ Institute of Crop Science, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture and Rural Affairs, Beijing, China.
² College of Agronomy, Shenyang Agricultural University, Shenyang, China.

Abstract

Grain filling is a critical process for achieving a high grain yield in maize (Zea mays L.), which can be improved by optimal combination with genotype and nitrogen (N) fertilization. However, the physiological processes of variation in grain filling in hybrids and the underlying mechanisms of carbon (C) and N translocation, particularly under various N fertilizations, remain poorly understood. The field experiment was conducted at Gongzhuling Farm in Jilin, China. In this study, two maize hybrids, i.e., Xianyu 335 (XY335) and Zhengdan958 (ZD958) were grown with N inputs of 0, 150, and 300 kg N ha^-1 (N0, N150, and N300) in 2015 and 2016. Results showed that the N application significantly optimized grain-filling parameters for both maize hybrids. In particular, there was an increase in the maximum filling rate (G _max ) and the mean grain-filling rate (G _mean ) in XY335 by 8.1 and 7.1% compared to ZD958 under the N300 kg ha^-1 (N300) condition, respectively. Simultaneously, N300 increased the small and big vascular bundles area of phloem, and the number of small vascular bundles in peduncle and cob at the milking stage for XY335. XY335 had higher root bleeding sap (10.4%) and matter transport efficiency (8.4%) of maize under N300 conditions, which greatly enhanced the ¹³C assimilates and higher C and N in grains to facilitate grain filling compared to ZD958. As a result, the grain yield and the sink capacity for XY335 significantly increased by 6.9 and 6.4% compared to ZD958 under N300 conditions. These findings might provide physiological information on appropriate agronomy practices in enhancing the grain-filling rate and grain yield for maize under different N applications, namely the optimization variety and N condition noticeably increased grain filling rate after silking by improving ear vascular structure, matter transport efficiency, and enhancing C and N assimilation translocation to grain, eventually a distinct improvement in the grain sink and the grain yield.

Keywords: 13C-photosynthates; grain filling; maize; matter transport efficiency; vascular bundle structure.