Sensitivity enhancement of magneto-optical Faraday effect immunoassay method based on biofunctionalized γ-Fe2O3@Au core-shell magneto-plasmonic nanoparticles for the blood detection of Alzheimer's disease

Kuen-Lin Chen; Ping-Huang Tsai; Chin-Wei Lin; Jian-Ming Chen; You-Jun Lin; Pradeep Kumar; Chien-Chung Jeng; Chiu-Hsien Wu; Li-Min Wang; Hsuan-Ming Tsao

doi:10.1016/j.nano.2022.102601

Sensitivity enhancement of magneto-optical Faraday effect immunoassay method based on biofunctionalized γ-Fe₂O₃@Au core-shell magneto-plasmonic nanoparticles for the blood detection of Alzheimer's disease

Nanomedicine. 2022 Nov:46:102601. doi: 10.1016/j.nano.2022.102601. Epub 2022 Sep 8.

Authors

Affiliations

¹ Institute of Nanoscience, National Chung Hsing University, Taichung, Taiwan; Department of Physics, National Chung Hsing University, Taichung, Taiwan. Electronic address: klchen@phys.nchu.edu.tw.
² Department of Neurology, National Yang Ming Chiao Tung University Hospital, Yilan, Taiwan.; Institute of Hospital and Health Care Administration, National Yang Ming Chiao Tung University, Taipei, Taiwan.
³ Graduate Institute of Applied Physics, National Taiwan University, Taipei, Taiwan.
⁴ Institute of Nanoscience, National Chung Hsing University, Taichung, Taiwan.
⁵ Department of Physics, National Chung Hsing University, Taichung, Taiwan.
⁶ Institute of Nanoscience, National Chung Hsing University, Taichung, Taiwan; Department of Physics, National Chung Hsing University, Taichung, Taiwan.
⁷ Division of Cardiology, National Yang Ming Chiao Tung University Hospital, Yilan, Taiwan.

PMID: 36089233
DOI: 10.1016/j.nano.2022.102601

Abstract

In this work, we conducted a proof-of-concept experiment based on biofunctionalized magneto-plasmonic nanoparticles (MPNs) and magneto-optical Faraday effect for in vitro Alzheimer's disease (AD) assay. The biofunctionalized γ-Fe₂O₃@Au MPNs of which the surfaces are modified with the antibody of Tau protein (anti-τ). As anti-τ reacts with Tau protein, biofunctionalized MPNs aggregate to form magnetic clusters which will hence induce the change of the reagent's Faraday rotation angle. The result showed that the γ-Fe₂O₃@Au core-shell MPNs can enhance the Faraday rotation with respect to the raw γ-Fe₂O₃ nanoparticles. Because of their magneto-optical enhancement effect, biofunctionalized γ-Fe₂O₃@Au MPNs effectively improve the detection sensitivity. The detection limit of Tau protein as low as 9 pg/mL (9 ppt) was achieved. Furthermore, the measurements of the clinical samples from AD patients agreed with the CDR evaluated by the neurologist. The results suggest that our method has the potential for disease assay applications.

Keywords: Alzheimer's disease; Faraday effect; Magneto-optical; Magneto-plasmonic; Nanoparticle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease* / diagnosis
Ferric Compounds
Gold
Humans
Immunoassay
Metal Nanoparticles
Nanoparticles*
tau Proteins

Substances

Ferric Compounds
Gold
tau Proteins
ferric oxide