A pilot and ex-vivo study of examination of endometrium tissue by catheter based optical coherence tomography

Bo Ding; Tao Jinyuan; Kuiyuan Tao; Zhenyang Ding; Shen Yang

doi:10.1186/s12880-022-00890-7

A pilot and ex-vivo study of examination of endometrium tissue by catheter based optical coherence tomography

BMC Med Imaging. 2022 Sep 10;22(1):162. doi: 10.1186/s12880-022-00890-7.

Authors

Bo Ding^#¹, Tao Jinyuan^#¹, Kuiyuan Tao², Zhenyang Ding³, Shen Yang⁴

Affiliations

¹ Department of Gynaecology and Obstetrics, The Affiliated Zhongda Hospital, Southeast University, Nanjing, 21009, China.
² School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China.
³ School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China. zyding@tju.edu.cn.
⁴ Department of Gynaecology and Obstetrics, The Affiliated Zhongda Hospital, Southeast University, Nanjing, 21009, China. shenyang@seu.edu.cn.

^# Contributed equally.

Abstract

Objective: This study aimed to distinguish ex-vivo normal and abnormal endometrium tissue samples histologically by catheter based optical coherence tomography (OCT).

Methods: A total of 72 ex-vivo endometrium specimens were obtained from June 2018 to March 2021 and were imaged fresh after hysterectomy. The scanned region of endometrium was excised for histological examination and endometrium OCT images were precisely compared to corresponding histological images. Meanwhile endometrium OCT images were analyzed quantitatively with intensity of backscattered light in region of interest (ROI) and maximum penetration depth of the OCT signal. Blinded qualitative analysis on endometrium OCT images was performed by 2 assessors to determine accuracy rate and inter-rating reliability on the histopathological diagnosis.

Results: OCT images were performed successfully in 72 endometrium specimens. Five endometrium specimens developed OCT interpretation criteria and the rest 67 endometrium specimens validated qualitatively and analyzed quantitatively. We defined an OCT criteria to distinguish normal endometrium and five different abnormal endometrium phases including proliferative endometrium, secretory phase endometrium, atrophic endometrium, endometrial hyperplasia with atypia and endometrial carcinoma based on OCT imaging features. The overall diagnosis accuracy achieved by the two assessors was 72.4% based on the OCT criteria. The inter-rater reliability between assessors on overall OCT images was substantial (Kendall τb of 0.720, p < 0.05). The changes in ROI minimum intensity, ROI maximum intensity, ROI average intensity and OCT signal maximum penetration depth of five different abnormal endometrium phases were significantly different (all p < 0.001). These parameters of endometrium carcinomas were significantly different from the other four endometrium phases (all p < 0.001).

Conclusion: OCT has the advantage of noninvasive and rapid diagnosis, which can contribute to the diagnosis of endometrial cancer and will be an indispensable complement to traditional biopsy. Future studies in vivo with larger samples are needed to confirm this conclusion.

Keywords: Endometrium; Gynecology; Optical coherence tomography; Uterus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biopsy
Catheters*
Endometrium / diagnostic imaging
Endometrium / surgery
Female
Humans
Reproducibility of Results
Tomography, Optical Coherence* / methods