Alginate based hydrogel inks for 3D bioprinting of engineered orthopedic tissues

Sumit Murab; Aastha Gupta; Małgorzata Katarzyna Włodarczyk-Biegun; Anuj Kumar; Patrick van Rijn; Patrick Whitlock; Sung Soo Han; Garima Agrawal

doi:10.1016/j.carbpol.2022.119964

Alginate based hydrogel inks for 3D bioprinting of engineered orthopedic tissues

Carbohydr Polym. 2022 Nov 15:296:119964. doi: 10.1016/j.carbpol.2022.119964. Epub 2022 Aug 5.

Authors

Sumit Murab¹, Aastha Gupta², Małgorzata Katarzyna Włodarczyk-Biegun³, Anuj Kumar⁴, Patrick van Rijn⁵, Patrick Whitlock⁶, Sung Soo Han⁴, Garima Agrawal⁷

Affiliations

¹ BioX Centre, School of Biosciences and Bioengineering, Indian Institute of Technology Mandi, Himachal Pradesh 175075, India.
² School of Chemical Sciences and Advanced Materials Research Centre, Indian Institute of Technology Mandi, Himachal Pradesh 175075, India.
³ University of Groningen, Zernike Institute for Advanced Materials, Polymer Science, Nijenborgh 4, 9747 AG Groningen, the Netherlands; The Silesian University of Science and Technology, Biotechnology Centre, Bolesława Krzywoustego 8, 44-100 Gliwice, Poland.
⁴ School of Chemical Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, South Korea.
⁵ University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, W. J. Kolff Institute, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
⁶ Division of Pediatric Orthopedic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA; Department of Orthopedic Surgery, College of Medicine, University of Cincinnati, Cincinnati, OH, USA; Department of Biomedical Engineering, College of Engineering and Applied Sciences, University of Cincinnati, Cincinnati, OH, USA.
⁷ School of Chemical Sciences and Advanced Materials Research Centre, Indian Institute of Technology Mandi, Himachal Pradesh 175075, India. Electronic address: garima@iitmandi.ac.in.

PMID: 36088004
DOI: 10.1016/j.carbpol.2022.119964

Abstract

3D printed hydrogels have emerged as a novel tissue engineering and regeneration platform due to their ability to provide a suitable environment for cell growth. To obtain a well-defined scaffold with good post-printing shape fidelity, a proper hydrogel ink formulation plays a crucial role. In this regard, alginate has received booming interest owing to its biocompatibility, biodegradability, easy functionalization, and fast gelling behavior. Hence, this review highlights the significance of alginate-based hydrogel inks for fabricating 3D printed scaffolds for bone and cartilage regeneration. Herein, we discuss the fundamentals of direct extrusion 3D bioprinting method and provide a comprehensive overview of various alginate-based hydrogel ink formulations that have been used so far. We also summarize the requirements of hydrogel inks and 3D printed scaffolds to achieve similarity to the native tissue environment. Finally, we discuss the challenges, and research directions relevant for future clinical translation.

Keywords: 3D bioprinting; Alginate; Bone; Cartilage; Scaffolds; Tissue engineering.

Publication types

Review

MeSH terms

Alginates
Bioprinting*
Excipients
Hydrogels
Ink
Printing, Three-Dimensional
Tissue Engineering
Tissue Scaffolds

Substances

Alginates
Excipients
Hydrogels