Characterization of KIR+CD8+ Regulatory T Cells in Humans by scRNA- and TCR-seq

Jing Li; Julie Wilhelmy; Mark M Davis

doi:10.1007/978-1-0716-2712-9_4

Characterization of KIR⁺CD8⁺ Regulatory T Cells in Humans by scRNA- and TCR-seq

Methods Mol Biol. 2022:2574:41-121. doi: 10.1007/978-1-0716-2712-9_4.

Authors

Jing Li¹, Julie Wilhelmy¹, Mark M Davis^{2

3

4}

Affiliations

¹ Institute of Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA, USA.
² Institute of Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA, USA. mmdavis@stanford.edu.
³ Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA. mmdavis@stanford.edu.
⁴ The Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA. mmdavis@stanford.edu.

Abstract

Previous studies have demonstrated the regulatory functions of Ly49⁺CD8⁺ T cells toward self-reactive CD4⁺ T cells in mice. Recently, we found KIR⁺CD8⁺ T cells are the equivalent of mouse Ly49⁺CD8⁺ T cells in humans. They are increased in patients with autoimmune or infectious diseases as a negative feedback mechanism to suppress the arising pathogenic cells and maintain peripheral tolerance. Here, we describe the methods on how we characterize the KIR⁺CD8⁺ T cells from different diseases using single-cell RNA and TCR sequencing.

Keywords: Autoimmune diseases; CD8+ regulatory T cells; Infection; Peripheral tolerance; Single-cell analysis.

MeSH terms

Animals
CD8-Positive T-Lymphocytes
Humans
Mice
RNA, Small Cytoplasmic*
Receptors, Antigen, T-Cell, alpha-beta / genetics
Single-Cell Analysis
T-Lymphocytes, Regulatory*

Substances

RNA, Small Cytoplasmic
Receptors, Antigen, T-Cell, alpha-beta

Abstract

MeSH terms

Substances

Grants and funding