In the uterus, the characteristics of smooth muscle contraction and the electrical activity that drives this contraction depends on hormonal cycles, and pregnancy status. Smooth muscle contraction is initiated by a change in membrane electrical potential, due to the flux of ions in and out of the intracellular space. Chains of action potentials throughout a section of muscle can result in coordinated contraction events. In this study, flexible printed circuit electrode arrays were applied to measure the bioelectric signals on the surface of a rat uterus in vivo. Variations in the electrical activity were quantified, including intermittent periods of activity and inactivity, which contain both slow-wave type activity (0.039 Hz ±0.017 Hz) and faster, spike-like activity (3.26 Hz ±0.27 Hz). The spike activity initiated at the ovarian end of the uterine horn, spreading towards the cervical end with a propagation velocity of 5.34 ± 2.32 mm [Formula: see text]. In conclusion, this pilot study outlines a new method of in vivo measurement of uterine electrical activity in rats. Clinical Relevance- Measurement of bioelectrical data using in vivo techniques provides insight into the electromechanical function of uterine smooth muscle, which could provide insights into what drives coordinated contraction in the uterus.