Wearable bioimpedance is a technique proposed to estimate breathing parameters such as respiratory rate (RR). However, its potential application lies in clinical investigation of daily-life activities like walking. This study evaluated the effect of the walking interference on the estimation of breathing parameters. 50 chronic obstructive pulmonary disease patients performed static and active measurements during thoracic bioimpedance acquisition. The static measurements included respiratory airflow for reference. The active measurements were used to estimate the walking interference from bioimpedance, and the obtained signals were added to static measurements for comparison with the reference. Afterward, we applied four different preprocessing methods to remove this walking interference and the resulting signals were used to detect the respiratory cycles and estimate breathing parameters (inspiratory time, expiratory time, duty cycle, and RR). The methods performed differently in terms of accuracy and mean average percentage error (MAPE), showing the need for specific preprocessing for active measurements. Furthermore, the MAPE values in the RR estimation were close to 3 % indicating that breathing parameters can be accurately estimated during walking. Accordingly, the present study reinforces the applicability of wearable bioimpedance for respiratory monitoring. Clinical relevance- This study exhibits the suitability of wearable bioimpedance to estimate accurate breathing param-eters during walking activities.