A smart tablet-phone-based system using dynamic light modulation for highly sensitive colorimetric biosensing

Hao Wang; Quchao Zou; Yuting Xiang; Jinhu Yang; Zhongyuan Xu; Wenjian Yang; Yue Wu; Jin Wu; Dong Liu; Ning Hu; Diming Zhang

doi:10.1016/j.talanta.2022.123862

A smart tablet-phone-based system using dynamic light modulation for highly sensitive colorimetric biosensing

Talanta. 2023 Jan 15:252:123862. doi: 10.1016/j.talanta.2022.123862. Epub 2022 Aug 24.

Authors

Hao Wang¹, Quchao Zou², Yuting Xiang³, Jinhu Yang¹, Zhongyuan Xu⁴, Wenjian Yang⁴, Yue Wu⁴, Jin Wu⁵, Dong Liu⁶, Ning Hu⁷, Diming Zhang⁸

Affiliations

¹ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China.
² The Second Affiliated Hospital Zhejiang University School of Medicine, Department of Clinical Medical Engineering, Hangzhou, 310009, China.
³ Department of Obstetrics and Gynecology, Affiliated Dongguan People's Hospital, Southern Medical University, Dongguan, 523059, China.
⁴ Research Center for Intelligent Sensing Systems, Zhejiang Laboratory, Hangzhou, 311100, China.
⁵ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China. Electronic address: wujin8@mail.sysu.edu.cn.
⁶ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China. Electronic address: liud99@mail.sysu.edu.cn.
⁷ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, 311200, China; Stoddart Institute of Molecular Science, Department of Chemistry, Zhejiang University, Hangzhou, 310027, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, China. Electronic address: huning@zju.edu.cn.
⁸ Research Center for Intelligent Sensing Systems, Zhejiang Laboratory, Hangzhou, 311100, China. Electronic address: zhangdm@zhejianglab.edu.cn.

PMID: 36084571
DOI: 10.1016/j.talanta.2022.123862

Abstract

Facile, efficient, and inexpensive biosensing systems are in high demand for biomedical test. In recent years, numerous smartphone-based biosensing systems have been developed to match demand for biomedical test in source-limited environment. However, application of these smartphone-based biosensing systems was limited because of performance gap between the smartphone-based systems and commercial plate readers. In this study, we have developed a smart tablet-phone-based colorimetric plate reader (STPCPR) with intelligent and dynamic light modulation for broad-range colorimetric assays. The STPCPR allows controllable modulation of exciting light in three different color channels that is lack in conventional smartphone-based system. Using optimized exciting modulation, the STPCPR shows higher sensitivities, lower detection limits, and broader detection ranges in test of pigments, proteins, and cells when compared to conventional plate readers and smartphone-based system. Therefore, the developed STPCPR can serve as an ideal next-generation smartphone-based biosensing system for point-of-care colorimetric test in diverse biomedical applications in source-limited environment.

Keywords: Biochemical test; Colorimetric assay; Light modulation; Microplate reader; Smartphone-based biosensing systems.

MeSH terms

Biological Assay
Biosensing Techniques*
Colorimetry*
Equipment Design
Point-of-Care Testing
Smartphone